Nanoneedle electroporation for CAR-Treg manufacturing
In Advanced Science, a scalable nanoneedle electroporation platform transfects primary human Tregs with CAR plasmids, a non-viral route to CAR-Treg production.
Viral transduction is currently the main route to clinical CAR-Treg production, but raises concerns over cost, immunogenicity and insertional mutagenesis. Ningjia Sun, Cong Wang, Will Edwards and colleagues introduce a GMP-compatible nanoneedle electroporation (nN-EP) platform that transfects HLA-A2-specific CAR plasmids into primary human Tregs with 43% efficiency.
Sun N, Wang C, Edwards W, … Chiappini C. Advanced Science (2025). doi:10.1002/advs.202416066